Long-term recovery dynamics determined by the degree of the disturbance - Ten years tracking of aquatic macroinvertebrate recolonisation after an industrial disaster (Red Sludge Disaster, Hungary).

A ten-year-long examination of macroinvertebrate community recovery was conducted following a catastrophic spill of highly alkaline red sludge (pH >13) into lowland streams. Our primary objective was to compare recovery patterns after coarse- and fine-grain disturbances, focusing on two aspects: i) trend analysis to reveal long-term changes of six community parameters, and ii) variation analyses to assess parameter changes over time. We conducted statistical analysis on long-term data series of macroinvertebrates obtained from quantitative samples collected at four sections with varying degrees of disturbance along the impacted stream sections. We developed a comprehensive theoretical framework comprising a series of sequential phases: Ramp-up, Overshoot, and Oscillation Phases. i) A trend analysis revealed that disturbances show a gradual recovery pattern, while variance analyses showed an asymptotic convergence to an equilibrium. ii) Evaluating these trends across phases unveiled that the initial recovery phase exhibited a steep trajectory, lasting 4-9 months, irrespective of disturbance severity. Coarse-grain disturbances induced a remarkable Overshoot phenomenon across all community metrics. The more severe the disturbance, the greater the height and duration of the Overshoot. Our results suggest that the presence or absence of Overshoot can serve as an indicator for coarse-grain disturbances in the context of large and infrequent disturbances (LID). The entire recovery process lasts for 2.5-3 years irrespective of the severity of the LID. In conclusion, a minimum survey duration of two and half years is deemed imperative to capture the phases of recovery, and changes associated with LID are not expected to extend beyond the three-year threshold. The theoretical framework, including Overshoot parameters, may assist future studies in comparing recovery patterns of different LID types. Furthermore, our theoretical framework is likely to be applicable to other groups of organisms given a sufficiently long monitoring of recovery, influenced also by the length of reproductive cycles.

Phytoplankton functional responses induced by extreme hydrological events in a tropical reservoir.

Climate change is affecting the global hydrological cycle, causing drastic changes in precipitation patterns. Extreme climatic events are becoming more frequent and intense than in the past, leading to water-level fluctuations and affecting aquatic ecosystems. Semiarid regions are very susceptible to changing climate. We analyzed a 10 years dataset from a tropical semiarid reservoir during extreme hydrological events (heavy rains and prolonged drought), and evaluated phytoplankton functional responses to environmental conditions. We found, as hypothesized, that phytoplankton functional structure change in a temporal scale due to water-volume fluctuation induced by the rainfall pattern. Depth and inorganic material acted as environmental filters selecting phytoplankton groups. High water level seems to improve water quality and low water level worsen it. Colonial and filamentous cyanobacteria dominate the wet period; however, it may have a critical threshold during severe periods of drought, which will lead to dominance of groups well adapted to low light conditions and with mixotrophic metabolism. Phytoplankton functional approaches can simplify phytoplankton identification and reflect better the environmental conditions than the taxonomic approach. Therefore, these approaches can help to understand the shifts in aquatic ecosystems under extreme hydrological events and predict functional response of phytoplankton being an important tool to water management and conservation. Supplementary Information: The online version contains supplementary material available at 10.1007/s10750-023-05241-3.

Winners and Losers: Cordulegaster Species under the Pressure of Climate Change.

(1) Bioclimatic factors have a proven effect on species distributions in terrestrial, marine, or freshwater ecosystems. Because of anthropogenic effects, the changes in these variables are accelerated; thus, the knowledge of the impact has great importance from a conservation point of view. Two endemic dragonflies, the Balkan Goldenring (Cordulegaster heros) and the Two-Toothed Goldenring (C. bidentata), confined to the hilly and mountainous regions in Europe, are classified as "Near Threatened" according to the IUCN Red List. (2) Modeling the potential occurrence of both species under present and future climatic conditions provides a more accurate picture of the most suitable areas. The models were used to predict the responses of both species to 6 different climate scenarios for the year 2070. (3) We revealed which climatic and abiotic variables affect them the most and which areas are the most suitable for the species. We calculated how future climatic changes would affect the range of suitable areas for the two species. (4) According to our results, the suitable area for Cordulegaster bidentata and C. heros are strongly influenced by bioclimatic variables and showed an upward shift toward high elevations. The models predict a loss of suitable area in the case of C. bidentata and a large gain in the case of C. heros.

Ecosystem services provided by marine and freshwater phytoplankton.

Phytoplankton, the ecological group of microalgae adapted to live in apparent suspension in water masses, is much more than an ecosystem's engineer. In this opinion paper, we use our experience as phytoplankton ecologists to list and highlight the services provided by phytoplankton, trying to demonstrate how their activity is fundamental to regulate and sustain Life on our Planet. Although the number of services produced by phytoplankton can be considered less numerous than that produced by other photosynthetic organisms, the ubiquity of this group of organisms, and their thriving across oceanic ecosystems make it one of the biological engines moving our biosphere. Supporting services provided by phytoplankton include almost half of the global primary and oxygen production. In addition, phytoplankton greatly pushes biogeochemical cycles and nutrient (re)cycling, not only in aquatic ecosystems but also in terrestrial ones. In addition, it significantly contributes to climate regulation (regulating services), supplies food, fuels, active ingredients and drugs, and genetic resources (provisioning services), has inspired artistic and craft works, mythology, and, of course, science (cultural services), and much more. Therefore, phytoplankton should be considered in all respects a true biosphere's engineer.

The influence of trophic status and seasonal environmental variability on morpho-functional traits in tropical man-made shallow lakes.

In the tropics, seasons are delimitated by the extent of rainfall resulting in seasonal differences in water parameters shaping phytoplankton community dynamics. Dry periods can intensify eutrophication and often result in seasonal or even perennial cyanobacterial dominance. This study was developed to evaluate phytoplankton response to trophic state and seasonal differences of environmental filters (dry and rainy periods) using the morphology-based functional groups (MBFG) approach. We also aimed at identifying environmental thresholds of each MBFG dominance in six man-made lakes located in the tropical semiarid region of Brazil. Our results showed clear MBFG association with lakes' trophic states. The dominant groups in mesotrophic conditions were members of MBFGs V (unicellular flagellates) and VI (non-flagellated with a siliceous exoskeleton), and in meso-eutrophic MBFG IV (medium size without specialized traits) dominated. Conversely, MBFG VII (with mucilage and aerotopes) and VIII (nitrogen-fixing cyanobacteria) dominated mostly under eutrophic conditions, though linked to shallower euphotic zones. Light and phosphorous were the most important environmental thresholds associated with MBFG's dominance. Overall, most of the lakes displayed seasonal differences in environmental filters. In contrast to what was expected, the rainy season was associated with higher nutrients, suspended solids, and reduced euphotic depth compared to the dry season. Our results, overall, show that the effects of seasonality varied across lakes and highlight eutrophication as the main environmental factor for MBFG selection suggesting reduced seasonality effects during dry years in the tropics.

Seasonality of parasitic and saprotrophic zoosporic fungi: linking sequence data to ecological traits.

Zoosporic fungi of the phylum Chytridiomycota (chytrids) regularly dominate pelagic fungal communities in freshwater and marine environments. Their lifestyles range from obligate parasites to saprophytes. Yet, linking the scarce available sequence data to specific ecological traits or their host ranges constitutes currently a major challenge. We combined 28 S rRNA gene amplicon sequencing with targeted isolation and sequencing approaches, along with cross-infection assays and analysis of chytrid infection prevalence to obtain new insights into chytrid diversity, ecology, and seasonal dynamics in a temperate lake. Parasitic phytoplankton-chytrid and saprotrophic pollen-chytrid interactions made up the majority of zoosporic fungal reads. We explicitly demonstrate the recurrent dominance of parasitic chytrids during frequent diatom blooms and saprotrophic chytrids during pollen rains. Distinct temporal dynamics of diatom-specific parasitic clades suggest mechanisms of coexistence based on niche differentiation and competitive strategies. The molecular and ecological information on chytrids generated in this study will aid further exploration of their spatial and temporal distribution patterns worldwide. To fully exploit the power of environmental sequencing for studies on chytrid ecology and evolution, we emphasize the need to intensify current isolation efforts of chytrids and integrate taxonomic and autecological data into long-term studies and experiments.

Global co-occurrence of methanogenic archaea and methanotrophic bacteria in Microcystis aggregates.

Global warming and eutrophication contribute to the worldwide increase in cyanobacterial blooms, and the level of cyanobacterial biomass is strongly associated with rises in methane emissions from surface lake waters. Hence, methane-metabolizing microorganisms may be important for modulating carbon flow in cyanobacterial blooms. Here, we surveyed methanogenic and methanotrophic communities associated with floating Microcystis aggregates in 10 lakes spanning four continents, through sequencing of 16S rRNA and functional marker genes. Methanogenic archaea (mainly Methanoregula and Methanosaeta) were detectable in 5 of the 10 lakes and constituted the majority (~50%-90%) of the archaeal community in these lakes. Three of the 10 lakes contained relatively more abundant methanotrophs than the other seven lakes, with the methanotrophic genera Methyloparacoccus, Crenothrix, and an uncultured species related to Methylobacter dominating and nearly exclusively found in each of those three lakes. These three are among the five lakes in which methanogens were observed. Operational taxonomic unit (OTU) richness and abundance of methanotrophs were strongly positively correlated with those of methanogens, suggesting that their activities may be coupled. These Microcystis-aggregate-associated methanotrophs may be responsible for a hitherto overlooked sink for methane in surface freshwaters, and their co-occurrence with methanogens sheds light on the methane cycle in cyanobacterial aggregates.

Effects of chronic sublethal progestogen exposure on development, reproduction, and detoxification system of water flea, Daphnia magna.

The presence of sex steroid hormones in aquatic ecosystems is of rapidly growing concern worldwide since they can affect the different non-target species including cladocerans. Although data are available on the effects of estrogens on the well-established ecotoxicological model organism Daphnia magna, the molecular or behavioural alterations induced by environmentally relevant concentrations (from a few ng L-1 to a few hundred ng L-1 in average) of progestogens have not been investigated on this species. In the present study, we exposed neonates of D. magna to relevant equi-concentrations (1, 10, 100, 500 ng L-1) of mixtures of four progestogens (progesterone, drospirenone, gestodene, levonorgestrel) in short-term (6 days) and long-term (21 days) experiments. Significant alterations were observed at the molecular, cellular, and individual levels. During the short-term exposure, all of the mixtures increased the gene expression of glutathione S-transferase (GST) detoxification enzyme, moreover, the activity of GST was also significantly increased at the concentrations of 10, 100, and 500 ng L-1. In long-term exposure, the number of days until production of the first eggs was reduced at the 10 ng L-1 concentration compared to control, furthermore, the maximum egg number per individual increased at the concentrations of 1 and 10 ng L-1. Based on the authors' best knowledge, this is the first study to investigate the effects of progestogens in mixtures and at environmentally relevant concentrations on D. magna. Our findings contribute to the understanding of the possible physiological effects of human progestogens. Future research should be aimed at understanding the potential mechanisms (e.g., perception) underlying the changes induced by progestogens.

Progestogen-induced alterations and their ecological relevance in different embryonic and adult behaviours of an invertebrate model species, the great pond snail (Lymnaea stagnalis).

The presence of oral contraceptives (basically applying estrogens and/or progestogens) poses a challenge to animals living in aquatic ecosystems and reflects a rapidly growing concern worldwide. However, there is still a lack in knowledge about the behavioural effects induced by progestogens on the non-target species including molluscs. In the present study, environmental progestogen concentrations were summarised. Knowing this data, we exposed a well-established invertebrate model species, the great pond snail (Lymnaea stagnalis) to relevant equi-concentrations (1, 10, 100, and 500 ng L-1) of mixtures of four progestogens (progesterone, drospirenone, gestodene, levonorgestrel) for 21 days. Significant alterations were observed in the embryonic development time, heart rate, feeding, and gliding activities of the embryos as well as in the feeding and locomotion activity of the adult specimens. All of the mixtures accelerated the embryonic development time and the gliding activity. Furthermore, the 10, 100, and 500 ng L-1 mixtures increased the heart rate and feeding activity of the embryos. The 10, 100, and 500 ng L-1 mixtures affected the feeding activity as well as the 1, 10, and 100 ng L-1 mixtures influenced the locomotion of the adult specimens. The differences of these adult behaviours showed a biphasic response to the progestogen exposure; however, they changed approximately in the opposite way. In case of feeding activity, this dose-response phenomenon can be identified as a hormesis response. Based on the authors' best knowledge, this is the first study to investigate the non-reproductive effects of progestogens occurring also in the environment on molluscan species. Our findings contribute to the global understanding of the effects of human progestogens, as these potential disruptors can influence the behavioural activities of non-target aquatic species. Future research should aim to understand the potential mechanisms (e.g., receptors, signal pathways) of progestogens induced behavioural alterations.

The global Microcystis interactome.

Bacteria play key roles in the function and diversity of aquatic systems, but aside from study of specific bloom systems, little is known about the diversity or biogeography of bacteria associated with harmful cyanobacterial blooms (cyanoHABs). CyanoHAB species are known to shape bacterial community composition and to rely on functions provided by the associated bacteria, leading to the hypothesized cyanoHAB interactome, a coevolved community of synergistic and interacting bacteria species, each necessary for the success of the others. Here, we surveyed the microbiome associated with Microcystis aeruginosa during blooms in 12 lakes spanning four continents as an initial test of the hypothesized Microcystis interactome. We predicted that microbiome composition and functional potential would be similar across blooms globally. Our results, as revealed by 16S rRNA sequence similarity, indicate that M. aeruginosa is cosmopolitan in lakes across a 280° longitudinal and 90° latitudinal gradient. The microbiome communities were represented by a wide range of operational taxonomic units and relative abundances. Highly abundant taxa were more related and shared across most sites and did not vary with geographic distance, thus, like Microcystis, revealing no evidence for dispersal limitation. High phylogenetic relatedness, both within and across lakes, indicates that microbiome bacteria with similar functional potential were associated with all blooms. While Microcystis and the microbiome bacteria shared many genes, whole-community metagenomic analysis revealed a suite of biochemical pathways that could be considered complementary. Our results demonstrate a high degree of similarity across global Microcystis blooms, thereby providing initial support for the hypothesized Microcystis interactome.

Trait-based diatom functional diversity as an appropriate tool for understanding the effects of environmental changes in soda pans.

Saline lakes, among the most seriously endangered ecosystems, are threatened due to climate change and human activities. One valuable feature of these environments is that they constitute areas of high biodiversity. Ecologists are, therefore, under great pressure to improve their understanding of the effects of natural and anthropogenic disturbances on the biodiversity of saline lakes. In this study, a total of 257 samples from 32 soda pans in Central Europe between 2006 and 2015 were examined. The effects of environmental variables and of geographical and limnoecological factors on functional diversity were analyzed. Furthermore, the explanatory power of the trait-based approach was assessed, and the applicability of the indices for biomonitoring purposes was determined. It was found that low habitat heterogeneity and harsh environments lead to the selection of a small number of suitable traits, and consequently, to a naturally low level of functional diversity. Anthropogenic activities enhance diversity at functional level due to the shift toward freshwater characteristics. On the regional scale, the effects of the region and status (natural, degraded, reconstructed) on diatom functional diversity were significant and more pronounced than that of the environmental and other limnoecological factors. The degree of variance found in functional diversity ascribed to environmental variables is five times greater in the case of the application of a trait-based approach, than when a taxonomic one is employed in the literature. Each of the tested functional diversity indices was sensitive to the most important environmental variables. Furthermore, these were type-specific and proved to be more complex indicators than taxonomic metrics. It is possible to suggest four functional diversity indices (FGR, FRic, FDis, and FDiv) which emphasize their independence from substrate and seasonal variations for ecological status assessment and conservation planning.

The impact of OTU sequence similarity threshold on diatom-based bioassessment: A case study of the rivers of Mayotte (France, Indian Ocean).

Extensive studies on the taxonomic resolution required for bioassessment purposes have determined that resolution above species level (genus, family) is sufficient for their use as indicators of relevant environmental pressures. The high-throughput sequencing (HTS) and meta-barcoding methods now used for bioassessment traditionally employ an arbitrary sequence similarity threshold (SST) around 95% or 97% to cluster sequences into operational taxonomic units, which is considered descriptive of species-level resolution. In this study, we analyzed the effect of the SST on the resulting diatom-based ecological quality index, which is based on OTU abundance distribution along a defined environmental gradient, ideally avoiding taxonomic assignments that could result in high rates of unclassified OTUs and biased final values. A total of 90 biofilm samples were collected in 2014 and 2015 from 51 stream sites on Mayotte Island in parallel with measures of relevant physical and chemical parameters. HTS sequencing was performed on the biofilms using the rbcL region as the genetic marker and diatom-specific primers. Hierarchical clustering was used to group sequences into OTUs using 20 experimental SST levels (80%-99%). An OTU-based quality index (IdxOTU) was developed based on a weighted average equation using the abundance profiles of the OTUs. The developed IdxOTU revealed significant correlations between the IdxOTU values and the reference pressure gradient, which reached maximal performance using an SST of 90% (well above species level delimitation). We observed an interesting and important trade-off with the power to discriminate between sampling sites and index stability that will greatly inform future applications of the index. Taken together, the results from this study detail a thoroughly optimized and validated approach to generating robust, reproducible, and complete indexes that will greatly facilitate effective and efficient environmental monitoring.

Functional redundancy modifies species-area relationship for freshwater phytoplankton.

Although species-area relationship (SAR) is among the most extensively studied patterns in ecology, studies on aquatic and/or microbial systems are seriously underrepresented in the literature. We tested the algal SAR in lakes, pools and ponds of various sizes (10-2-108 m2) and similar hydromorphological and trophic characteristics using species-specific data and functional groups. Besides the expectation that species richness increases monotonously with area, we found a right-skewed hump-shaped relationship between the area and phytoplankton species richness. Functional richness however did not show such distortion. Differences between the area dependence of species and functional richness indicate that functional redundancy is responsible for the unusual hump-backed SAR. We demonstrated that the Small Island Effect, which is a characteristic for macroscopic SARs can also be observed for the phytoplankton. Our results imply a so-called large lake effect, which means that in case of large lakes, wind-induced mixing acts strongly against the habitat diversity and development of phytoplankton patchiness and finally results in lower phytoplankton species richness in the pelagial. High functional redundancy of the groups that prefer small-scale heterogeneity of the habitats is responsible for the unusual humpback relationship. The results lead us to conclude that although the mechanisms that regulate the richness of both microbial communities and communities of macroscopic organisms are similar, their importance can be different in micro- and macroscales.

Decoupling of active and passive reasons for the invasion dynamics of Aedes albopictus Skuse (Diptera: Culicidae): Comparisons of dispersal history in the Apennine and Florida peninsulas.

Aedes albopictus is an important vector of several diseases including dengue- and Chikungunya fever and is a potential vector of Zika-fever. The invasion dynamics of Aedes albopictus was reconsidered by comparing the temperature-related development of the mosquito with the observed real geographical distribution in Florida and in Italy. The potential number of generations and the annual dispersal distances of the mosquito were calculated for the estimates. The estimated total dispersals are 3.6-4.6 km/year/generation in Italy and 4.6-5.3 km/year/generation in Florida, values that are at least five to six times higher than those derived from release and recapture studies and from the previously measured flying distances of female Asian tiger mosquitoes. Subtracting the calculated dispersal distances with the known active dispersal of female Ae. albopictus, the passive dispersal component of the total dispersal distances was found to be 2.8-4.1 km/year/generation in Italy and 3.8-4.8 km/year/generation in Florida. Our results confirm that the active dispersal of female mosquitoes plays a secondary role in determining the rate of areal expansion and, in contrast, passive factors may play a primary role. It was concluded, based on similar average values of the passive dispersal distances of the mosquito in Florida and Italy, that at large spatial scales the anthropogenic component can be well estimated.

Taxonomy- or trait-based ecological assessment for tropical rivers? Case study on benthic diatoms in Mayotte island (France, Indian Ocean).

Diatom-based ecological quality assessment methods have been implemented and used regularly in the Water Framework Directive. These indices use the species' abundance profiles along a specific environmental gradient, which they aim to assess. However, this approach has several problematic issues including the unstable and fast-changing diatom taxonomy. The use of traits can be a solution if their responses to the environmental pressure are well-defined. Here, we developed taxonomy-based and trait-based diatom assemblage indices to assess the ecological status of riverine sites on a tropical island. The two indices are based on two sub-indices that measure the diatom assemblage response to a nutrient and organic matter/turbidity gradient. Both taxonomy- and trait-based indices correlated significantly with the selected environmental gradients of the test database, which was not used during index development. We showed that traits could be used for quality assessment of the Mayotte rivers and require much less effort than taxonomy-based indices. There were differences between the two types of indices, which are discussed in this paper. As a perspective for further studies, tests of trait-based indices among different eco-regions would be challenging.

Presence of potential toxin-producing cyanobacteria in an oligo-mesotrophic lake in Baltic Lake District, Germany: an ecological, genetic and toxicological survey.

Massive developments of potentially toxic cyanobacteria in Lake Stechlin, an oligo-mesotrophic lake in the Baltic Lake District of Germany raised concerns about toxic contamination of these important ecosystems. Field samples in the phase of mass developments of cyanobacteria were used for genetic and toxicological analyses. Microcystins and microcystin genes were detected in field samples of the lake for the first time. However, the toxins were not produced by the dominant taxa (Dolichospermum circinale and Aphanizomenon flos-aquae) but by taxa, which were present only in low biomass in the samples (Microcystis cf. aeruginosa and Planktothrix rubescens). The phytoplankton successions during the study period revealed an increase of cyanobacterial populations. The findings contribute to the changes that have been investigated in Lake Stechlin since the mid-1990s. The possible reasons behind these developments may be climate change, special weather conditions and an increased nutrient pool.

Leaf litter decomposition in Torna stream before and after a red mud disaster.

The aim of the study was to estimate the breakdown of the allochthonous litter in an artificial stream running in an agricultural area and compare it with the same values following a toxic mud spill into the same stream. Litter bags were filled with three types of leaves (Quercus robur, Populus tremula and Salix alba) and placed to the bottom of the river. Ergosterol was used to detect fungal biomass. We supposed the absence of fungi and the retardation of leaf litter decomposition. Only pH and conductivity increased significantly. Leaf mass loss after the catastrophe was much slower than in 2009 and the decay curves did not follow the exponential decay model. Prior to the catastrophe, leaf mass loss was fast in Torna, compared to other streams in the area. The reason is that the stream is modified, the bed is trapezoid and covered with concrete stones. Fungal biomass was lower, than in the pre-disaster experiment, because fungi did not have enough leaves to sporulate. Leaf mass loss followed the exponential decay curve before the disaster, but after that it was possible only after a non-change period.

What drives the distribution of the bloom-forming cyanobacteria Planktothrix agardhii and Cylindrospermopsis raciborskii?

The cyanobacteria Planktothrix agardhii and Cylindrospermopsis raciborskii are bloom-forming species common in eutrophic freshwaters. These filamentous species share certain physiological traits which imply that they might flourish under similar environmental conditions. We compared the distribution of the two species in a large database (940 samples) covering different climatic regions and the Northern and Southern hemispheres, and carried out laboratory experiments to compare their morphological and physiological responses. The environmental ranges of the two species overlapped with respect to temperature, light and total phosphorus (TP); however, they responded differently to environmental gradients; C. raciborskii biovolume changed gradually while P. agardhii shifted sharply from being highly dominated to a rare component of the phytoplankton. As expected, P. agardhii dominates the phytoplankton with high TP and low light availability conditions. Contrary to predictions, C. raciborskii succeeded in all climates and at temperatures as low as 11 °C. Cylindrospermopsis raciborskii had higher phenotypic plasticity than P. agardhii in terms of pigments, individual size and growth rates. We conclude that the phenotypic plasticity of C. raciborskii could explain its ongoing expansion to temperate latitudes and suggest its future predominance under predicted climate-change scenarios.

Succession of phytoplankton functional groups regulated by monsoonal hydrology in a large canyon-shaped reservoir.

Liuxihe reservoir is a deep, monomictic, oligo-mesotrophic canyon-reservoir in the subtropical monsoon climate region of southern China. Phytoplankton functional groups in the reservoir were investigated and a comparison made between the succession observed in 2008, an exceptionally wet year, and 2009, an average year. The reservoir shows strong annual fluctuations in water level caused by monsoon rains and artificial drawdown. Altogether 28 functional groups of phytoplankton were identified, including 79 genera. Twelve of the groups were analyzed in detail using redundancy analysis. Because of the oligo-mesotrophic and P-limited condition of the reservoir, the dominant functional groups were those tolerant of nutrient (phosphorus) deficiency. The predominant functional groups in the succession process were Groups A (Cyclotella with greatest axial linear dimension<10 µm), B (Cyclotella with greatest axial linear dimension>10 µm), LO (Peridinium), LM (Ceratium and Microcystis), E (Dinobryon and Mallomonas), F (Botryococcus), X1 (Ankistrodesmus, Ankyra, Chlorella and Monoraphidium) and X2 (Chlamydomonas and Chroomonas). The development of groups A, B and LO was remarkably seasonal. Group A was dominant during stratification, when characteristic small size and high surface/volume ratio morphology conferred an advantage. Group LO was dominant during dry stratification, when motility was advantageous. Group B plankton exhibited a high relative biomass during periods of reduced euphotic depth and isothermy. Groups LM, E, F, X1 and X2 occasionally exhibited high relative biomasses attributable to specific environmental events (e.g. drawdown, changes in zooplankton community). A greater diversity of phytoplankton functional groups was apparent during isothermy. This study underscores the usefulness of functional algal groups in studying succession in subtropical impoundments, in which phytoplankton succession can be significantly affected by external factors such as monsoonal hydrology and artificial drawdown, which alter variables such as retention time, mixing regime and thermal structure and influence light and nutrient availability.